Bio-Rad GS-800™ Calibrated Densitometer - Manual (Page 19)

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The Quantity One software returns a "Calibration error " or similar message when
attempting to calibrate.
1. Check if the OD values for the Transparency target in the EDIT CALIBRATION
window are entered properly.
2. Make sure the calibration windows are clean and free of dust, dirt, fluid, and other
contaminants that can obstruct the path of light.
3. Check for correct offset value. For the GS-800 Calibrated Imaging Densitometer the
correct offset value is 00.
4. Make sure that the transparency scan image shows up correctly at the bottom of the
Quantity One acquisition window and that during the calibration the software
identifies each step wedge with a number inside a yellow rectangle in the sequence
shown in the photo below:
1 2 3 4 5 6 ……………and so on.
CORRECT CALIBRATION SCAN WINDOW LABELING
The Quantity One software returns a "Densitometer not ready" or similar message.
1. Verify the power connection to the Densitometer.
2. Check that the power switch is turned on.
3. Check the Installation procedure (Section 3), to see if you followed the instructions.
4. Verify that the GS-800 imaging device is selected in the FILE menu of the Quantity
One software.