Bio-Rad GS-800™ Calibrated Densitometer User Manual

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Section 1 General Information

1.1 Introduction

The Model GS-800 Calibrated Imaging Densitometer is a high performance, calibrated
imaging densitometer that converts transparent and opaque electrophoretic samples into
digital data. These data are analyzed with Quantity One® software operating in Microsoft
Windows® computer systems. For a step-by-step guide to scanning and analyzing images,
please refer to your QuantityOne software user manual.


The main features of the Model GS-800 Calibrated Imaging Densitometer include:

• Adjustable resolution from 36.3 microns to 127.0 microns;

• Reflectance and transmittance

• Scanning area of 29 cm by 40 cm

• Sampling rate of up to 700 dots per inch (dpi).

• Variable wavelength filter architecture for improved color discrimination

• Analog to digital conversion at full 12-bit accuracy

• Calibrated transmission tablet to ensure accurate OD readings

• Reflective gray scale tablet to ensure reproducible reflectance values

• Supported by Quantity One software, which provides versatile image display,

optimization, and quantitation features

• USB interface

1.2 Installation Qualification and Operational Qualification (IQ/OQ)


The calibration of the Model GS-800 Calibrated Imaging Densitometer can be validated by
following the GS-800 Calibrated Densitometer Installation Qualification and Operational
Qualification protocols. These protocols are available as an accessory to the GS-800
Calibrated Imaging Densitometer (Catalog number 170-7956) these straightforward and
simple to follow IQ/OQ protocols include instructions for the installation of the hardware
and software, and instructions for the verification of the reflectance and transmittance
calibration functions.

Following proper installation, a linear dynamic range of 3.0 OD for the
Transmissive scanning and 2.0 OD for Reflectance scanning can be confirmed with
greater than 95% confidence. For additional details, contact your local Bio-Rad
Representative.

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