Bio-Rad Human Metabolic and Hormone Assays - Manual (Page 10)

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1. Plan Plate Layout
Determine the total number of wells in the experiment using the Plate
Layout Template on page 29 or the Plate Formatting tab in Bio-Plex
Manager
™ software. A suggested plate layout is shown in Figure 2, with
all conditions in duplicate.
1. Assign standards to columns 1 and 2, with the highest
concentration in row A and the lowest concentration in row H.
2. Assign the blank to wells A3 and A4. The blank should consist of
standard diluent alone and be processed in the same manner as
sample and standard wells. Bio-Plex Manager software automatically
subtracts the assay blank (B) MFI value from all other assay wells.
3. User-specific controls, as well as the quality controls supplied in the
kits, are assigned to wells in columns 3 and 4.
4. The remainder of the plate is available for samples.
Legend
S Standards
B Blank
X Samples
C Controls
Fig. 2. Suggested plate layout. For detailed instructions on
plate formatting in Bio-Plex Manager, see section Read Plate.