Bio-Rad Human Metabolic and Hormone Assays - Manual (Page 15)

13
5. Prepare Samples
The kit has sufficient reagents to run 37 samples in duplicate for a full plate
analysis. General guidelines for preparing samples are provided below. For
more information, contact Bio-Rad Technical Support.
Serum and Plasma
Serum, EDTA plasma, or citrate plasma are the preferred sample types for
these assays. Heparin-treated plasma, while compatible with these assays,
may absorb certain soluble proteins of interest.
n
Avoid using hemolyzed samples, as this may lead to false results.
n
Freezing samples at –70°C immediately after preparation and keeping
samples frozen until use should provide adequate protection from
degradation. Avoid multiple freeze and thaw cycles.
n
Once thawed, keep samples on ice.
n
Do not freeze diluted samples