Bio-Rad Bio-Plex® Assay Builder - Manual (Page 11)

5. Then select the Plate Groupings tab to display the plate grouping
tools.
a) Select Group and drag the cursor across all the wells to
define each assay group (exclude the ones that contain lysates
provided with the assays).
b) The first well of the group is automatically assigned as the
Reference well and the remaining wells in the group are Member
wells. To change the Reference well, select Reference and
click on the new Reference well.
c) Select Member/Reference or Reference/Member from the Ratio
pull-down list. When selecting the more common Member/Reference
option, the ratio of fluorescence intensity of each well will be
calculated against the fluorescence intensity of the Reference well.
This value will be reported as a ratio in the results file.
6. Select Step 6 (Enter Sample Info) and enter sample information. This
is the location where the wells are identified as containing either
Bio-Plex phosphoprotein or total target assays (see the example on
the right).
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Plate Grouping Example
2. Select OK and follow the instructions for CAL1 and CAL 2
calibration.
Prepare Protocol
1.
Open a new protocol by selecting File, then New from the main
menu. Locate the steps at the left of the protocol menu.
2. Select Step 1 (Describe Protocol) and enter information about the assay.
3. Select Step 2 (Select Analytes) and select the panel for
Phosphoproteins or Total Targets. Choose the target proteins for all
the assays on the plate. If both phosphoprotein and total target
assays are run on the same plate, two separate protocols must be
entered.
4. Select Step 3 (Format Plate) and click on the Plate Formatting tab.
Click on and drag the cursor over all the wells that contain
lysates.
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Plate Formatting Example