Bio-Rad Bio-Plex® Assay Builder - Manual (Page 3)

Section 1
Introduction
Bio-Plex phosphoprotein assays and Bio-Plex total target assays are
bead-based multiplex assays (Luminex xMAP technology) that detect the
phosphorylation of proteins in lysates derived from cell culture or tissue
samples. These 96-well plate-format assays allow profiling of the specific
phosphorylation state of up to 100 different proteins using as few as two
wells and as little as 25 µl of lysate per well. The Bio-Plex total target
assay reports the abundance of the target protein in one well, while the
Bio-Plex phosphoprotein assay reports the level of phosphorylation of that
protein in a separate well. These instructions apply to both assays. For a
current list of all Bio-Plex phosphoproteins and total target assays, visit
www.bio-rad.com/products/phosphoproteins.
Available as Singleplex or Premixed Multiplex Assays
Bio-Plex phosphoprotein and total target assays are available as
singleplex or premixed multiplex assays. Singleplex assays are designed
to be flexible. They can be used individually to test for a single
phosphoprotein at a time, or they can be combined to create a multiplex
assay to test for a specific set of phosphoproteins in a single sample.
Premixed multiplex assays are the more convenient format for repeat
testing of a specific set of phosphoproteins. Both the coupled beads and
the detection antibodies are premixed and quality tested at Bio-Rad.
These assays are only available through Bio-Rad’s online x-Plex assay
service (www.bio-rad.com/bio-plex/x-plex/).
For research use only. Not for diagnostic procedures.
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