Bio-Rad Mouse Typer Isotyping Kit - Manual (Page 4)

1.2 Materials Required
Kit Components:
Catalog Quantity/
Number Product Description Package
172-2055 Mouse Typer Sub-Isotyping Panel , includes 10 ml each
ultra pure rabbit anti-mouse subclass specific
anti-serum to mouse IgG
1
, IgG2
a
, IgG2
b
, IgG
3
,
IgM, IgA,
χ
chain, and
λ
chain.
172-1019 EIA Grade Affinity Purified Goat Anti-Rabbit IgG 1 ml
(H + L), human adsorbed, horseradish peroxi-
dase conjugate (GAR-HRP).
172-1064 Peroxidase Substrate System , contains 2,2’- 200 ml
Azino-di (3-ethyl-benzthiazoline sulfonate [6])
and hydrogen peroxide.
1.3 Storage and Stability
Temperature Shelf Life
Mouse Typer Sub-Isotyping Panel 4 °C 1 year
GAR-HRP Antibody Conjugate 4 °C 1 year
Peroxidase Substrate System 4 °C >1 year
1.4 Reagents and Equipment Not Included
1. Specific mouse monoclonal antibody, culture or ascites fluid,
and its cognate antigen.
2. Sodium chloride (NaCl), sodium phosphate dibasic hepta-
hydrate (Na
2
HPO
4
-7H
2
O), sodium phosphate monobasic
(NaH
2
PO
4
-H
2
O), ACS Reagent Grade.
3. Tween 20, EIA Grade (Bio-Rad catalog number 170-6531).
4. Bovine Serum Albumin BSA (Sigma catalog number A 9647).
5. Thimerosal, (Sigma catalog number T 5125).
6. Oxalic acid, dihydrate (J. T. Baker catalog number 0230-1).
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