Bio-Rad BioLogic LP System - Manual

1
The Gradient Mixer proportions two buffers as commanded by either the BioLogic LP Controller or the
Econo Gradient Pump and mixes the buffers in a dynamic mixing chamber to form a gradient. This
valve may be used with either the BioLogic LP system or the Econo Gradient Pump; both applications
are shown below.
Figure 1. Gradient Mixer
I Use with the BioLogic LP
Plumbing and Operation of the Gradient Mixer
1. To attach the Gradient Mixer to the system rack:
a. Ensure that the clamping screw is loose.
b. Clip the attaching bracket to a convenient place on the rack.
c. Tighten the clamping screw.
d. Connect the signal cable from the Gradient Mixer to the port marked “Gradient Mixer” on the
rear of the BioLogic LP Controller.
2. Plumb the Gradient Mixer’s inlet ports to the solvent reservoirs.
Using barb-to-male luers, connect an appropriate length of tubing between buffer ports A and B to
their respective solvent reservoirs (see Figure 2.) Match the appropriate luer connector with tubing
ID, and ensure a firm connection but do not overtighten. Similarly, connect the gradient mixer to
either port A/B of the LP Buffer Select Valve or directly to the pump inlet if the Buffer Select valve
is not used.
3. If a Method includes gradient formation, then the Gradient Mixer’s proportioning valve will
automatically toggle between the two positions to create the gradient.
If a Method calls for either 100% A or B buffer, then the corresponding port will remain open.
The stir bar in the Gradient Mixer’s mixing chamber will activate whenever the BioLogic LP pump
is running.