Bio-Rad BioLogic LP System - Manual (Page 4)

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II Use with the Econo Gradient Pump
Plumbing and Operation of the Gradient Mixer
1. To attach the Gradient Mixer to the system rack:
a. Ensure that the clamping screw is loose.
b. Clip the attaching bracket to a convenient place on the rack.
c. Tighten the clamping screw.
d. Connect the signal cable from the Gradient Mixer to the port marked “Mixer” on the rear of the
Econo Gradient Pump.
2. Using barb-to-male luers, connect an appropriate length of tubing between buffer ports A and B to
their respective solvent reservoirs (see Figure 4.) Match the appropriate luer connector with tubing
ID, and ensure a firm connection but do not overtighten. Similarly, connect the gradient mixer to the
pump inlet.
3. If a Program includes gradient formation, then the Gradient Mixer’s proportioning valve will
automatically toggle between the two positions to create the gradient.
If a Program calls for either 100% A or B buffer, then the corresponding port will remain open.
The stir bar in the mixing chamber activates when the Econo Gradient Pump is running.
Figure 4. Use with the Econo Gradient Pump