Bio-Rad BioLogic LP System - Manual (Page 2)

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Figure 2. Use with the BioLogic LP
Care of the Gradient Mixer’s Proportioning Valve
Normally, the only maintenance required is to flush the valve with water. Use the BioLogic LP’s Manual
mode to set the buffer to 50%B and place both inlet tubes in a vessel of water and pump until the salt is
washed from the valve. Do not leave high salt buffers in the valve, as the crystallized salt may damage
the valve. For long-term storage, store the valve in 20% EtOH. Do not expose the valve to pressures
greater than 20 psi.
Care of the Gradient Mixer’s Mixing Chamber
To clean the mixing chamber of residue, such as salt:
1. Disconnect the Gradient Mixer’s cable from the BioLogic LP Controller.
2. Use a 3/8” socket driver or box wrench to carefully remove the top of the mixer by rotating the top
counterclockwise. Refer to Figure 3.
3. The magnetic stir bar may be removed from the mixing chamber by turning the unit upside down
and tapping the unit in the palm of your hand until the stir bar drops out of the chamber.
4. The mixing chamber may now be cleaned as required.
5. The chamber may be flushed by connecting a luer-tipped syringe to the “A” port and slowly passing
fluid through the valve and mixing chamber.