Bio-Rad Bio-Gel P Polyacrylamide Gel - Manual (Page 12)

Fig. 4. Placement and positioning of GST buffers.
After the buffer bottles are installed, the cartridges are ready to insert
into the instrument, and are automatically equilibrated by the
preprogrammed methods. Insert the cartridges as described in Section 2.2
and as shown in Figure 2. After use, the cartridges should be stored at
4°C and be reused within 6 months.
Section 5
Desalting and Cartridge Cleaning Buffer
Kit
5.1 General Buffer and Cartridge Information
The buffers and solutions provided in the desalting and cartridge
cleaning buffer kit consist of a PBS-based protein desalting buffer,
cartridge cleaning buffers, and a cartridge storage solution. The PBS
solution provides an optimized ionic strength to prevent nonspecific
interactions between proteins and the matrix, and is a commonly used
buffer for the storage of a wide variety of proteins. Note: desalting and
dartridge dleaning buffers are already included in Profinia™
native IMAC and GST purification and buffer kits
. It can be used to
purify other proteins with MBP- or Strep-tag, using the Profinia system
(see Bulletin 5741 and 5744.) Optimized desalting and storage solutions
for unique proteins will need to be determined empirically. The desalting
solutions and cartridges can be used to remove or exchange salts, buffers,
and small molecules from any protein sample. Table 5 provides a list of
buffer compositions.
The desalting cartridges are provided in 20 mM Bis-Tris, pH 6.5 with
0.05% sodium azide as a preservative, and can be stored between 4 and
22°C prior to use. The cartridges are packed with 10 ml of Bio-Rad's
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