Bio-Rad Bio-Gel P Polyacrylamide Gel - Manual (Page 14)

conjunction with a desalting cartridge. A syringe and a 3-way stopcock
combination are used to fill the desalting loop. The desalting loop accessory
comes ready to use in two sizes, 2 ml and 10 ml. For ordering information,
refer to Section 6.
Fig. 6. Desalting sample loop and fittings.
Determine the appropriate combination of desalting loop and desalting
cartridge for the protein sample volume to be applied. For a 2 ml sample,
choose the 2 ml desalting loop and the 10 ml desalting cartridge. For a
10 ml sample, choose the 10 ml desalting loop and the 50 ml desalting
cartridge. Application of a sample volume smaller than the desalting loop
volume is not recommended, as it will result in increased dilution of the
sample and reduced desalting resolution.
After cartridge lines are automatically primed, the sample loop and
desalting cartridge can be installed. The desalting loop accessory is
designed to connect to the instrument in the cartridge 1 position, while the
desalting cartridge is placed in the cartridge 2 position. Refer to Figure 6
for the proper installation of a 2 ml desalting loop and 10 ml desalting
cartridge.
To connect and fill the desalting loop:
1. Insert the male luer fitting from cartridge holder 1 into the top female
luer fitting of the 3-way stopcock. Ensure that the second female luer
fitting of the 3-way stopcock is facing out so the syringe may be easily
inserted.
2. Connect the male luer of the end of the desalting loop to the female
luer fitting of cartridge holder 1.
3. To apply sample, fill the syringe and insert it into the open female luer
of the 3-way stopcock. Turn the 3-way stopcock lever down, towards
the loop, and fill the loop with sample, avoiding bubbles as much as
possible.
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