Specifications – Bio-Rad Profinia Protein Purification System Buffers and Reagents User Manual
Page 2
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Specifications
Quantity
45 g, 90 g
Description
Profinia urea packs contain
high-purity chromatography
grade urea which is ideal for
solubilizing and denaturing
proteins in E. coli extracts.
Storage
Store between 4°C and 22°C
Instructions
Add the entire contents of the
for use
90 g pack to the 176 ml of
Profinia Denaturing IMAC
Lysis Buffer (#620-0209) or
Profinia Denaturing IMAC
Wash Buffer 1 (#620-0210) to
make 250 ml of a 6 M urea
solution. Add the entire contents
of the 45 g pack to the 88 ml of
Profinia Denaturing IMAC
Wash Buffer 2 (#620-0211) or
Profinia Denaturing IMAC
2
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