Bio-Rad ENrich™ High-Resolution Ion Exchange Columns - Manual (Page 6)

2 ENrich Instruction Manual
Table 1. Column characteristics.
Q S
Column dimensions
(diameter x height, mm)
5 x 50 10 x 100 5 x 50 10 x 100
Column volume, ml 1 8 1 8
Maximum protein capacity, mg
Bovine serum albumin 130 1000 – –
Human IgG – – 120 940
Average particle size, μm 10 10 10 10
Maximum operating pressure,
psi
500 500 500 500
Recommended flow
rate, ml/min*
1 4 1 4
* At room temperature. Viscosity may increase at lower temperatures, which will
reduce the recommended flow rates.
1.3 Connection to the NGC and Other
Chromatography Systems
The ENrich columns are fitted with 10-32 type female fittings on either
end. Standard 10-32 fittings can be used to plumb the column to
Bio-Rad’s NGC chromatography system or other vendors’ systems.
Adaptors are available for connection to systems that use 1/4-28
fittings (catalog # 750-0564).