Bio-Rad Bio-Scale™ CHT™ Type I Columns - Manual (Page 9)

Fig. 2. Instructions for removal of top frit.
1. Remove the upper and lower end fittings from the column.
2. Remove the upper lock nut from the top end cap.
3. Unscrew the bottom end cap from the protective shield and slide out the glass column.
4. Pull out the upper bed support from the glass column.
5. Using the frit removal tool, remove the distribution screen and frit by pressing the hook
into the frit in a sideways motion with slight downward pressure.
6. Add 5 ml of buffer to the top of the resin bed. Place a new frit and screen into the tube.
Insert the upper bed support and use it to carefully push down the frit and screen until it
just touches the top of the resin bed.
7. Insert the glass column through the protective shield, into the top end-cap and align the
bed support prong with the top end-cap guide. Attach the bottom end-cap to the shield.
Insert the lock nut into the top end-cap and twist down until the plunger is at the top of the
bed.
8. Attach column to a pump and pump buffer at 2.0 ml/min for 5 to 10 minutes. Inspect the
column for voids and adjust accordingly.
7
FLOW
Upper end fitting
Upper lock nut
Top end cap
Protective shield
Upper bed support
Distribution screen
Top frit
Glass column
Bottom end cap
Lower end fitting