Bio-Rad Prepacked Gravity Flow Columns for Ion Exchange - Manual (Page 5)

2. Remove the column cap to allow the eluant
to flow freely.
3. Snap off the seal at the bottom of the
column.
4. Monitor the level of liquid in the reservoir,
and do not allow the resin to become dry.
5. Rinse the resin with several bed volumes
(at least 2 ml) of the sample solvent.
Discard rinse.
6. Add sample.
7. Collect effluent.
8. To reduce the flow rate or stop the flow,
use a Luer 3-way stopcock or the yellow
end-cap.
Section 4
Ion Exchange Capacity
The resin packing determines the ion
exchange capacity of each prefilled Poly-Prep
column. Table 1 lists the ion exchange capacity
of the resin in each prefilled Poly-Prep column.
Table 1. Ion Exchange Capacity of
Prefilled Poly-Prep Columns
Ion Exchange Capacity
Resin (nominal meq/2 ml of rein)
AG ® 1-X8 resin 2.4
AG 2-X8 resin 2.4
AG 50W-X4 resin 2.2
AG 50W-X8 resin 3.4
Chelex ® 100 resin 0.8
Bio-Rex ® 70 resin 4.8
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