Bio-Rad Bio-Gel P Polyacrylamide Gel User Manual
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Section 1
Introduction
The Econo-Pac 10DG columns allow quick and
economical desalting and buffer exchange of a wide
range of samples. The columns are packed with Bio-Gel
®
P-6DG gel. This polyacrylamide gel allows high
recovery, without interference from biologically active
material leaching from the matrix.
In desalting, a chromatography matrix is chosen for
its ability to exclude the solutes of interest from the pores
of the gel, and to retain the smaller contaminants. The
Econo-Pac 10DG columns are packed with a matrix
which excludes solutes greater than 6,000 daltons,
allowing them to elute in the void volume. Therefore,
desalting occurs between the excluded components larger
than 6,000 daltons and the included components smaller
than 6,000 daltons.
Desalting is not a capacity dependent separation; the
efficiency is not limited by the concentration of the
sample. Therefore the only limiting parameter is sample
volume, which is limited to the void volume of the
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