Bio-Rad Bio-Gel P Polyacrylamide Gel - Manual (Page 9)

Section 7
Product Information
Catalog
Number
Product Description
732-2010 Econo-Pac 10DG Desalting Columns, 50
732-6000 Bio-Spin 6 Chromatography Columns, 10
732-6002 Bio-Spin 6 Chromatography Columns, 25
732-6004 Bio-Spin 30 Chromatography Columns, 10
732-6006 Bio-Spin 30 Chromatography Columns, 25
150-0738 Bio-Gel P-6DG Gel, 100 G
150-0739 Bio-Gel P-6DG Gel, 1 KG
732-1010 Empty Econo-Pac 10 Columns (50), include
caps and bed support
732-1015 Econo-Pac 10 Rack, 12 place acrylic rack
731-7005 Poly Column Rack, holds 10 Econo-Pac
columns
732-8102 2-way Stopcock, 10
12 13
5.3 Application 2: Removal of Affi-Gel ®
or Affi-Prep ® MAPS ® II Binding Buffer
from Antibody Solutions
1. Follow steps 1 through 3 in the general desalting
procedure. (Note: The equilibration buffer should
contain a minimum of 10 mM NaCI for IgG
monoclonal antibodies, or 50 mM NaCI for IgM
monoclonal antibodies.)
2. Load up to 3.0 ml of the elution fraction collected
from the Affi-Gel or Affi-Prep protein A column.
3. Add 8.0 ml of a suitable buffer to elute the antibody.
Collect 1.0 ml fractions.
4. Analyze the fractions to determine which fractions
to pool.
Section 6
Storage Conditions
Econo-Pac 10DG columns should be stored at room
temperature in 0.02% sodium azide.
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