Bio-Rad Bio-Gel P Polyacrylamide Gel - Manual (Page 5)

Most buffers are suitable for desalting. The low
residual charge of Bio-Gel P-6DG gel makes deionized
water a good choice, since salts are not needed to
overcome any ionic interactions between the gel and the
solute. However, before desalting or exchanging a
sample into a buffer, the solubility of the sample in the
new buffer should be tested. For example, most
antibodies require an ionic strength of at least 10 mM to
remain in solution. IgM antibodies require as much as
40 mM to stay soluble.
Section 5
Procedures
Three procedures are included in this section. The
first procedure is for general desalting or buffer
exchange. The other two procedures are for specific
applications.
5
Section 3
Column Specifications
Bed volume 10 ml
Total column volume 30 ml
Maximum sample volume 3.3 ml
Recommended sample volume 3.0 ml
Void volume 3.3 ml
Packing buffer 10 mM sodium phosphate,
10 mM NaCI, pH 7.0, with
0.02% sodium azide
Flow rate range 1.1 - 1.5 ml/min
Column material Polypropylene
Frit material Polyethylene
Desalting gel Bio-gel P-6DG gel
pH range 2 - 10
Operating temperature range 2 - 45 °C
Section 4
Buffer Considerations
The Econo-Pac 10DG columns are shipped
containing sodium azide as a preservative. Before using
the column, remove this buffer and equilibrate the
column in a suitable buffer for desalting.
4
LIT81D 7/9/98 8:36 AM Page 4