Bio-Rad Aminex Organic Acid and Alcohol Analysis Columns - Manual (Page 3)

should be set at 0.1-0.2 ml/minute while
the cartridge is being inserted. To fit the
cartridge into the holder, remove one end
of the holder. Loosen the other end a few
turns. Insert the cartridge into the holder
with firm pressure. The cartridge will fit
into the holder securely. Fit the free end of
the holder onto the cartridge and screw it
down. With the Deashing Holder, the cen-
ter fitting should be placed between the
two cartridges in the holder. The holder
ends need only be screwed down finger-
tight. Further tightening is unnecessary,
and may damage the seal or holder. Never
use tools to tighten this seal.
Temperature and
Pressure Limits
The maximum temperature for the
holder is 60 °C. System pressure should
not exceed 4,000 psi.
Leakage
If the cartridge holder leaks at less
than its rated pressure limit, do not try to
force a seal. A leak indicates that the seals
are either dirty, scratched, or damaged. If
the cartridge holder leaks, the seals should
be cleaned or replaced (Catalog number
125-0147).
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must be cut, file or grind the tubing end as
necessary so that the end cut is perpendic-
ular to the tubing axis, and so that the
internal diameter has not been constricted.
Fig. 1. Connections to the holder.
Use 1/16" 316 stainless steel tubing,
0.010" ID, to connect the holder. See
Figure 1. Tighten the assembly of tubing,
ferrule, and nut finger-tight. Be sure the
tubing end bottoms squarely in the holder
fitting. Using a wrench, tighten the nut 3/4
turn past finger-tight. The ferrule will be
permanently set against the tubing. The
connection only needs to be tight enough
to seal, and should not be over-tightened.
Repeat this procedure for the other end of
the cartridge holder.
Cartridge Installation
Prior to inserting the cartridge, all air
should be removed from the tubing. After
all air bubbles are removed, the flow rate
2
Nut Ferrule
Holder
1/16" stainless steel tubing
M1250131B 11/28/2000 3:16 PM Page 4