Bio-Rad Affi-Gel Hz Hydrazide Gel - Manual (Page 13)

Chaotropic Elution
4 M NaSCN
6 M urea
5 M guanidine-HC1
Elution Strategies
The preceding eluants generally do not denature antibodies. *
Eluant must be compatible with the antigen. Several methods should
be tried in the following order:
1. Acid (pH 2-3.5) is common. May cause inactivity of some pro-
teins. Can reduce solubility of IgG.
2. Chaotropic salts (3.5 M NaSCN, 3-6 M GuHCl) are often effec-
tive. Usually used at neutral pH. SCN should not be used with
low pH.
*Minimize the exposure to harsh conditions by neutralizing acid quickly and dilut-
ing chaotropes.
Section 6
Production Information
6.1 Affi-Gel Hz Hydrazide Gel Product
Specifications
Operating temperature range 2-30 °C
Elution pH operating range 2-11
Particle size range 75-300 µm hydrated
6.2 Ordering Information
Catalog
Number
Product Description
153-6060 Affi-Gel Hz Immunoaffinity Kit
153-6047 Affi-Gel Hz Hydrazide Gel, 25 ml
153-6054 Affi-Gel Hz 10x Coupling Buffer, 500 ml
153-6055 Affi-Gel Hz Oxidizer (NaIO
4
), 250 mg
732-2010 Econo-Pac 10DG Desalting Columns, 30
153-7307 DEAE Affi-Gel Blue Gel, 100 ml
153-6159 Affi-Gel Protein A MAPS II Kit
156-0006 Affi-Prep Protein A Support, 5 ml
156-0005 Affi-Prep Protein A Support, 25 ml
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