Bio-Rad Affi-Gel Hz Hydrazide Gel - Manual (Page 2)

Table of Contents
Section 1 Introduction...............................................................1
1.1 Immunoaffinity Coupling and Usefulness of the
Immobilized Antibody.........................................................1
1.2 Hydrazide Coupling Chemistry ...........................................2
1.3 Diagram of Kit Components ...............................................2
Section 2 Monoclonal Antibodies.............................................3
Section 3 Antibody Purification ...............................................3
Section 4 Immobilization Protocol...........................................4
4.1 Buffer Exchange ..................................................................4
4.2 Oxidation of IgG..................................................................6
4.3 Desalting
Procedure.............................................................7
4.4 Coupling of Oxidized IgG to Affi-Gel Hz
Hydrazide Gel......................................................................7
Section 5 Applications of Affi-Gel Hz Immobilized IgG........9
5.1 Conditioning the Immunoaffinity Column ..........................9
5.2 Sample
Application ...........................................................10
5.3 Elution Suggestions ...........................................................10
Section 6 Product Information ...............................................11
6.1 Specifications.....................................................................11
6.2 Ordering
Information.........................................................11
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