Bio-Rad Affi-Gel Hz Hydrazide Gel - Manual (Page 4)

1.2 Hydrazide Coupling Chemistry
1.3 Diagram of Kit Components
Materials and Equipment Not Included in this Kit
Essential
15 ml screw cap test tubes - polypropylene or polystyrene, leak-free
End-over-end rotating mixer
Transfer pipets
pH meter
UV-visible spectrophotometer
2
Sugar residue of
carbohydrate on the
Fc region of IgG. Periodate oxidation
of vicinal hydroxyls
to form aldehydes. Oxidized IgG specific
coupling to Affi-Gel
Hz hydrazide gel.
HOH
2
C HOH
2
C
NalO
4
HOH
2
C
OH OH
OH OH OH
NH
N N
NH
O O
IgG IgG IgG
Affi-Gel Hz
gel
Affi-Gel Hz
gel
Econo-Pac ® 10DG
Desalting Columns
1 x 10 cm
Econo-Column ®
Chromatography
Column
Affi-Gel Hz 10 x
Coupling Buffer, 25 ml
Affi-Gel Hz Oxidizer,
25 mg NalO
4
each
Affi-Gel Hz Hydrazide
Gel, 5 ml
LIT101C 9/1/98 9:39 AM Page 2