Bio-Rad Bio-Rex® Reactor Grade Resins - Manual (Page 3)

Instructions for Use
The batch method or the column method may be used
to deionize a sample with mixed bed resin.
Batch Method
The batch method is the addition of resin directly into
the sample followed by stirring to achieve deionization.
1. Weigh out about 5 g of fresh resin for every 100 ml of
sample.
2. Add resin to sample and stir or shake for 1 hour.
3. Filter or decant sample from resin.
Note: If samples contain sugars or polyhydric alcohols, it
is important to use the minimum quantity of resin required
for deionization, and to remove the resin from the sample
after the deionization process to minimize adsorption of
the sample. If the sample contains extremely hydrophilic
proteins, the resin should be placed in dialysis tubing to
avoid direct contact of the resin with the sample. If the
anion resin beads change from blue to gold prior to deion-
ization, the quantity of resin should be increased.
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Table 1. Mixed Bed Resin Specifications
Bio-Rex
AG 501-X8 AG 501 -X8 (D) MSZ 501(D)
Resin Resin Resin
Chemical H + & OH - H + & OH - H + & OH -
form
Resin type Mixed bed Mixed bed Mixed bed
Physical form Light and dark Blue and gold Blue and brown
gold beads beads beads
Minimum total 1.5 meq/ml 1.5 meq/ml 1.5 meq/ml
capacity
Particle 300-1,180 µm 300-1,180 µm 600±100 µm
diameter
Mesh size 20-50 20-50 25-35
Matrix Styrene divinyl- Styrene divinyl- Styrene divinyl-
benzene benzene benzene
All of the mixed bed resins are used for deionization.
Deionization can be performed by exchanging the solute
cations for hydrogen on the resin and the solute anions for
hydroxyl on the resin. The resulting neutralization yields
water.
Resin-H + Resin-OH + NaCl → Resin-Na + Resin-Cl + H
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LIT205B 6/17/98 12:15 PM Page 2