Bio-Rad Media Sampler Pack - Manual (Page 3)

1
Section 1. Product Description
CFT ceramic fluoroapatite (Ca
5
(PO
4
)
3
F)
2
is an inorganic calcium phosphate used in
the chromatographic separation of biomolecules. CFT has separation
characteristics similar to CHT
™ ceramic hydroxyapatite, but can be used under
chromatographic conditions as low as pH 5 to separate acidic proteins.
CFT is a rigid spherical macroporous chromatographic support that has been
sintered at high temperatures to modify its microcrystalline lattice into a fused
ceramic. The sintering process improves the mechanical strength of the particle. It
is a composite of fluoroapatite and hydroxyapatite prepared by chemically
converting hydroxyapatite nanocrystals with a fluorine reagent. The molecular
weight of CFT is 1,008.6. The solubility constant is approximately 1.0 x 10
–60 . CFT
is available in two distinct material types, Type I, sintered at 400ºC, and Type II,
sintered at 700ºC, and one particle size, 40 µm. The two types are chemically
identical, but have been sintered at different times and temperatures, resulting in a
physically and chemically stable support.
CFT Type I and II behave similarly to their CHT counterparts except in the case of
IgG. CFT Type II has a higher dynamic binding capacity for IgG than CFT Type I,
whereas CHT Type I has a higher dynamic binding capacity for IgG than CHT Type
II. Otherwise, the protein selectivity of these two products is comparable. Solubility
or apatite lifetime will be less of a concern with CFT when lower pH buffer systems
are required. In order to determine which material types provide optimal
chromatographic performance, it is beneficial to evaluate both CFT and CHT, and
both Types I and II.
4110183A.qxd 6/7/2004 9:07 AM Page 4