Bio-Rad Media Sampler Pack - Manual (Page 5)

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1.2 Chemical Compatibility
Solution Compatibility Time
6 M guanidine-HCl Yes >24 hr
8 M urea Yes >24 hr
2 N NaOH Yes >24 hr
100% ethanol or methanol Yes >24 hr
1% SDS Yes >24 hr
Chelating agents No >24 hr
Chelating buffers (e.g., PIPES) No >24 hr
Organic acids/salts (e.g., malate, citrate, acetate) Caution >24 hr
Tris-HCl,* pH 7–9 Yes >14 days
MES,** pH 5–6.7 Yes >14 days
NaOAc,** pH 4.2–-5.2 Yes >14 days
* Not known to dissolve CHT ceramic hydroxyapatite
** Known to dissolve CHT ceramic hydroxyapatite
Solubility tests were carried out to determine if CFT could be stored in common starting buffers that are
known to affect the solubility of CHT. Calcium content was determined by inductively coupled plasma-mass
spectrometry (ICP-MS) on CFT stored at 37°C for 14 days in the indicated buffers.
1.3 General Handling
CFT is a rigid support that can operate under high flow rates and pressures.
Particles can be fractured through mechanical stress and handling, e.g.:
• Stirring with magnetic stirrers
• Operating at too high a speed or for too long with axial or radial stirrers
• Recycling slurries with peristaltic or diaphragm pumps
If fines are created, they can cause an increase in the column backpressure and
may reduce binding capacity.
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