Bio-Rad Media Sampler Pack - Manual (Page 4)

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1.1 Characteristics of CFT Ceramic Fluoroapatite
Characteristics Types I and II
Functional groups Ca 2 +, PO
4
3 -, F-
Dynamic binding capacities*
Bovine IgG (Type I) 14–17 mg/ml
Bovine IgG (Type II) 30–32 mg/ml
Lysozyme (Type I) 25–30 mg/ml
Lysozyme (Type II) 17–21 mg/ml
Surface area
Type I 33–36 m 2 /g
Type II 16–18m 2 /g
Maximum operating pressure 800 psi or 55 bar or 5.5 MPa
Nominal mean particle size (µm) 40 ± 4
Nominal density 0.79–0.85 g/ml
Recommended linear flow rate 50–300 cm/hr
Operating pH range 5–14
Storage pH range 11–14
Regeneration 3–5 column volumes (CV) of the following after every run:
Normal 400 mM sodium phosphate, pH 6.8
Difficult 400 mM trisodium phosphate, pH 11–12
Basic proteins (pI > 7) 1–2 N NaCl or KCl, pH 10–12
Cleaning-in-place (CIP) Same as regeneration and/or any of the following:
Lipids 1 CV H
2
O, then 3–5 CV 70–100% methanol,
ethanol, isopropanol, or acetonitrile, then 1 CV H
2
O
1% SDS
Other 6 M urea or 6 M guanidine-HCl
Sanitization Perform a CIP, then 3–5 CV 1–2 N NaOH or KOH;
exposure time =1 hr
Recommended column storage 0.1–1 N NaOH or KOH
Shelf life (dry, unused material) >2 years
* Dynamic binding capacity conditions:
Samples
1 mg/ml bovine IgG or 2 mg/ml lysozyme in binding buffer (QB
1%
determination)
Column volume 5.0 ml
Flow rate Determined on a 1.1 x 5.2 cm column and run at 150 cm/hr
Binding buffer 10 mM sodium phosphate, pH 6.8
Elution buffer 400 mM sodium phosphate, pH 6.8
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