Bio-Rad Bio-Gel P Polyacrylamide Gel User Manual
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Section 1
Introduction
Bio-Gel P gels are porous polyacrylamide beads prepared by
copolymerization of acrylamide and N,N'-methylene-bis-acrylamide.
The gels are extremely hydrophilic and essentially free of charge,
and provide efficient, gentle gel filtration of sensitive compounds.
Their synthetic composition and freedom from soluble impurities
preclude eluate contamination. High resolution is assured by consis-
tent narrow distribution of bead diameters and excellent molecular
weight discrimination.
Bio-Gel P gel is compatible with dilute organic acids, 8 M urea,
6 M guanidine-HCl, chaotropic agents, reducing agents such as dithio-
threitol and mercaptoethanol, and detergents such as SDS, CHAPS,
and Triton
®
X-100. Bio-Gel P gel may be used effectively with dis-
tilled water however, buffers of > 50 mM ionic strength are recom-
mended for most protein separations.
Miscible organic solvents may be added to the eluants used with
Bio-Gel P gel. Alcohol up to 20% will not substantially alter the
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