Bio-Rad Bio-Gel P Polyacrylamide Gel - Manual (Page 5)

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Table 2. Properties of Bio-Gel P-Gels
Typical
Typical Fractionation
Particle Size Hydrated Bed Range/Nominal
Range, Hydrated Volume, ml/g Typical Flow Exclusion Limit
Gel Beads (µM) of Dry Gel Rates (cm/hr)* (Daltons)**, †
Bio-Gel P-2 Gel, Fine 45-90 3 5.0-10 100-1,800
Bio-Gel P-2 Gel, Extra Fine < 45 <10 100-1,800
Bio-Gel P-4 Gel, Medium 90-180 4 15-20 800-4,000
Bio-Gel P-4 Gel, Fine 45-90 10.0-15 800-4,000
Bio-Gel P-4 Gel, Extra Fine < 45 <10 800-4,000
Bio-Gel P-6 Gel, Medium 90-180 6.5 15-20 1,000-6,000
Bio-Gel P-6 Gel, Fine 45-90 10.0-15 1,000-6,000
Bio-Gel P-6 Gel, Extra Fine < 45 <10 1,000-6,000
Bio-Gel P-6DG Gel 90-180 6.5 15-20 1,000-6,000
Bio-Gel P-10 Gel, Medium 90-180 7.5 15-20 1,500-20,000
Bio-Gel P-10 Gel, Fine 45-90 10.0-15 1,500-20,000
Bio-Gel P-30 Gel, Medium 90-180 9 7.0-13 2,500-40,000
Bio-Gel P-30 Gel, Fine 45-90 6.0-11 2,500-40,000
Bio-Gel P-60 Gel, Medium 90-180 11 4.0-6 3,000-60,000
Bio-Gel P-60 Gel, Fine 45-90 3.0-5 3,000-60,000
Bio-Gel P-100 Gel, Medium 90-180 12 4.0-6 5,000-100,000
Bio-Gel P-100 Gel, Fine 45-90 3.0-5 5,000-100,000
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* Flow rates determined in a 1.5 x 70 cm
column, using a hydrostatic pressure
head:bed of 1:1.
** Fractionation ranges above 40,000 dal-
tons are for globular molecules.
† For quality control purposes, the exclu-
sion limits are determined by calculating
the Kd, or distribution coefficient. The
distribution coefficient is a measure of
the residence time of a molecule in the
pores of the gel, and is expressed as:
(V
e
- V
o
)/(V
t
- V
o
), where V
e
is the elution
volume of the individual proteins, V
o
is
the void volume and V
t
is the total avail-
able volume measured by a small
molecule such as vitamin B
12
.
LIT174B 10/13/98 9:26 AM Page 4