Bio-Rad Aurum™ Total RNA 96 Kit User Manual
Page 22
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18
Problem
Possible Cause
Recommended
Solution
Total RNA prep
Incorrect use of wash
Add the appropriate
performs poorly in solutions
volume of 95–100%
downstream
ethanol to the low
applications
stringency wash
solution before initial
use
RNA is degraded
See problem “RNA
degradation”
Ethanol contamination in Add 1–3 min to the
prep (eluate volumes
purge time after the final
>60 µl)
wash step
Blot RNA binding plate
drip directors with paper
towels; ensure that the
plate underside is dry
Inefficient drying
Dry for a full 4 min and
ensure no droplets of
wash buffer remain on
the walls or the bottom
of the plate
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