Bio-Rad Fluorescent Assay Kits - Manual (Page 2)

Section 1 Introduction ..................................................................1
Section 2 Kit Components ............................................................1
2.1 Contents ......................................................................................1
2.2 Materials
Required ....................................................................2
2.3 Storage and Stability ..................................................................2
Section 3 Preparation of Solutions ..............................................2
Section 4 Experimental Procedures ............................................3
4.1 Fluorometer Set-Up ....................................................................3
4.2 Sample Measurement Guidelines ..............................................3
4.3 Standard Curve Preparation........................................................4
4.4 ß-galactosidase Assay ................................................................4
4.5 Reading Samples ........................................................................5
4.6 Data Analysis..............................................................................6
Section 5 Appendix ........................................................................8
5.1 Microplate Assay........................................................................8
5.2 Microplate Fluorometer Guidelines............................................8
Section 6 References ......................................................................8
Section 7 Additional Supplies ......................................................9
Table of Contents
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