Bio-Rad Fluorescent Assay Kits - Manual (Page 7)

method parameters shown in Table 3. The Shutdown Method
included in the BioFocus software can be used as a template to
quickly program the dsDNA Shutdown Method.
Table 3. dsDNA Shutdown Method
ID: END Description: terminates autosequence
Prep 1: Pre-Inject from [WATER ] to [Waste ] 180 sec
Prep 2: Pre-Inject from [NITROGEN ] to [Waste ] 300 sec
No Injection
Polarity: Negative -> Positive
Run Voltage: 0.00 kV
Current Limit: 0.30 mA
Inlet buffer: [WATER ]
Outlet buffer: [WATER ]
Cartridge
Temperature:
20 degrees Celsius
Run Time: 1.00 min
Detector: LIF
Channel: 1
Laser(s) turned off at the end of the run group
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Table 2. dsDNA Analysis Method for 24 cm Capillary
Section 4
System Shutdown Method
Drying of the buffer at the ends of the capillary may cause
the capillary to become plugged. For short term storage (24 hours),
leave the capillary filled with the CE dsDNA run buffer and place
the capillary ends in test tubes filled with deionized water at 4 °C.
For long term storage, the capillary should be flushed thoroughly
with water and then purged with nitrogen. This may be programmed
to run automatically at the end of an automation sequence using the
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Set the carousel temperature to 20 °C
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