Recommended binding and washing solutions – Bio-Rad Chromatographic Surfaces User Manual
Page 4
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Recommended Binding and Washing Solutions
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ProteinChip H50 buffer (catalog #K20-00001)
(10% acetonitrile, 0.1% trifluoroacetic acid (TFA))
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0–50% methanol or acetonitrile ± 0.1–1% TFA
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0.1% TFA may be added to the binding solution to
increase binding
Fractionation for Serum Profiling
Fractionation of serum prior to profiling is recommended. The
fractionation procedure produces 6 fractions containing proteins
separated on the basis of their isoelectric point. Fractionation
allows segregation of highly abundant proteins into a limited
number of fractions, thereby reducing signal suppression effects
on lower-abundance proteins.
© 2006 Bio-Rad Laboratories, Inc.
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