Bio-Rad ProteinChip Serum Fractionation Kits - Manual (Page 7)

© 2006 Bio-Rad Laboratories, Inc. © 2006 Bio-Rad Laboratories, Inc.
50,000 100,000 150,000 200,000
0
1.0
0
0.5
1.0
0
1.0
0
1.0
0
0.2
0.4
0.6
0
1.0
2.0
3.0
Fraction 6
Fraction 5
Fraction 3
Fraction 2
Fraction 1
Fraction 4
1.5
0.5
0.5
0.5
1.5
4.0
Fig. 2. Example fractionation of human serum sample. Profiled on
ProteinChip CM10 arrays (20–200 kD).
5,000 10,000 15,000 20,000
0
10
20
30
40
0
5.0
10
0
1.0
2.0
3.0
0
2.5
5.0
7.5
0
10
20
30
0
25
50
75
Fraction 6
Fraction 5
Fraction 3
Fraction 2
Fraction 1
Fraction 4
7.5
2.5
Fig. 1. Example fractionation of human serum sample. Profiled on
ProteinChip CM10 arrays (2–20 kD).
Appendix A: Performance Specification
When the ProteinChip serum fractionation kit is used according to
this protocol, each sample processed will produce six fractions
(see Figure 1).
In
te
n
s
it
y
40
30
20
10
0
10
7.5
5.0
2.5
0
3.0
2.0
1.0
0
7.5
5.0
2.5
0
30
20
10
0
75
50
25
0
Fraction 1
Fraction 2
Fraction 3
Fraction 4
Fraction 5
Fraction 6
5,000 10,000 15,000 20,000
m/z
5,000 10,000 15,000 20,000
Fraction 1
Fraction 2
Fraction 3
Fraction 4
Fraction 5
Fraction 6
1.0
0.5
0
1.5
1.0
0.5
0
1.0
0.5
0
1.5
1.0
0.5
0
0.6
0.4
0.2
0
4.0
3.0
2.0
1.0
0
m/z
In
te
n
s
it
y