Bio-Rad Fish DNA Barcoding Kit - Manual (Page 6)

4 Fish DNA Barcoding Quick Guide
15. Remove the spin column from the 2 ml
microcentrifuge tube, discard the flowthrough
at the bottom of the 2 ml tube, and replace
the spin column in the same tube. Centrifuge
columns for a full 2 min to remove residual
traces of ethanol and dry out the matrix.
16. Label two clean 2 ml capless microcentrifuge
tubes with your fish sample name and your
initials.
17. When your 2 min spin is completed, remove
the spin columns and discard the 2 ml
microcentrifuge wash tubes.
18. Place the spin column for each sample into a
new capless 2 ml microcentrifuge tube from
step 16.
19. Using a fresh pipet tip for each sample, add
100 µl of distilled water to each spin column,
being careful not to touch the resin. Elute the
DNA by centrifuging for 1 min at full speed.
20. Label two clean 2 ml microcentrifuge tubes (with
caps) Fish 1 and Fish 2 and your initials.
21. Transfer the eluted DNA into the appropriately
labeled 2 ml microcentrifuge tube with caps
and store the DNA at 4°C until you are ready to
proceed.
Initials Initials
1
2
1
1
Clean 2 ml tube,
labeled with “1”
and initials
Dist.
Water
100 µl
1 2
Initials Initials
1 2
14,000 x g
2 min
1 2
1 2
1 2
1