Spinning samples – Iris Sample Processing StatSpin® CytoFuge 12 Versatile Cytocentrifuge System User Manual
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Spinning Samples
CAUTION
– Maintain a 300 mm (11.8 inches) clearance envelope around cytocentrifuge during
operation. Do not deposit any potentially hazardous materials within the clearance envelope.
CAUTION
– Do not lean, or place materials, on the cytocentrifuge.
CAUTION
– The seals in the removable rotor are intended to stop gross fluid leakage only. These
seals should not be relied upon for protection against biohazardous contamination.
CAUTION
– Follow Universal Precautions with all biological specimens, regardless of whether the
specimen is known to contain an infectious agent. Bioseals cannot be relied upon as a sole
safeguard against contamination by pathogens. Always load and unload the rotor under a biological
safety hood.
Loading
1.
Press Stop/Open button on front panel to release latch and open cover.
2.
Remove rotor from cytocentrifuge and transport to a biological safety hood.
3.
Remove the lid and load the concentrators into the rotor (concentrators must be equally
distributed and equally filled to properly balance rotor).
4.
Slowly add sample to the bottom of the Filter Concentrator or Cell concentrator well. Avoid
getting droplets onto the walls of the funnel/well.
5.
Install lid onto rotor, press center button to lock.
6.
Place rotor into cytocentrifuge, assure rotor is fully seated onto cone in center of bowl.
7.
Close cover and set spin cycle.
CAUTION
– Do not overfill the concentrators. Addition of excess amounts of liquid to Cell or Filter
Concentrators will result in the fluid being “spun out” during centrifugation.
CAUTION
– While in the resting position, the rotor is designed to maintain the Concentrators at an
angle to prevent fluid from contacting the slide and/or the filter card prior to spinning. Keep the rotor
level during transport to and from the cytocentrifuge to ensure proper concentrator angle.
Running a Cycle
1.
Use the buttons on the front panel to select the desired acceleration, speed and time for the
cycle. Alternatively, select a program number to retrieve previously saved settings (See
Programming - pg 8). The table below lists the relative centrifugal force (RCF) at several
speeds.
2.
Press start to begin cycle. The Cytofuge 12 will spin for the set time and automatically stop.
Note: Settings cannot be changed once the cycle begins.
Speed
(RPM)
RCF
(xg)
Speed
(RPM)
RCF
(xg)
200
6
1200
204
300
13
1300
240
400
23
1400
278
500
36
1500
319
600
51
1600
364
700
70
1700
410
800
91
1800
460
900
115
1900
513
1000
142
2000
568
1100
172
Note: Relative centrifugal force at other speeds may be calculated by:
RCF = 142 x (RPM/1000)
2