BUCHI NIRFlex N-500 - Manual (Page 21)

4 Description of function
21 NIRFlex N-500 Operation Manual, Version F
4 .2 .1 Transflectance mode
Translucent and opaque liquids can be analyzed via transflectance mode. The light penetrates the
liquid, is diffusely reflected by the reference plate and passes the sample a second time. The trans-
flected rays contain the spectral information of the sample.
Factors that can influence the measurement:
Not enough sample material available •
Air bubbles in the measurement path and under the transflectance cover •
Sample not homogeneous or representative •
Temperature of sample material •
Sample cup or transflectance cover inadequate (e.g. cup material and thickness, blind spots, dirt •
etc.)
NIRFlex Solids:
Diffuse reflecting / reference plate
Small, defined gap for liquid samples
Detector
NIR light
Sample cup / petri dish
NIRFlex Fiber Optic Solids / SMA:
Diffuse reflecting / reference plate
Small gap for liquid samples
Fiber optics (example)