ChemoMetec YC-100 - Manual (Page 23)

2 Introducing the NucleoCounter
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A fluorescent image of the content of the measurement chamber is thus . Example of
such an image is shown in Figure 4. Each spot in the image represents a cell containing
DNA stained with PI.
Figure Figure Figure Figure 4 44 4. Fluorescent image (from NucleoView PC software) of cell DNA contained in
the measurement chamber of a NucleoCassette
2.1.2 Built-in image analysis
The NucleoCounter is equipped with advanced software for image analysis. This integral
software analyzes the recorded image and counts the number of nuclei in the image. As
the volume of the cassette measurement chamber and the dilution of the lyzate is
known, the cell density in the initial sample can be calculated. The concentration of
cells in the initial sample is presented in the NucleoCounter display. If the optional
software (NucleoView) is used, the results and the recorded fluorescent image can be
viewed.
The entire process takes approximately 30 seconds, from pressing “ Run” to the
presentation of the result in the display of the NucleoCounter.
Performing a cell count with the NucleoCounter is objective and the results are
independent of the operator.
2.2 The NucleoCassette
The NucleoCassette is a custom made disposable plastic device designed for optimal
sample handling and safe disposal. The cassette is shown in figure 5. The main features
of the NucleoCassette are the piston, the flow system containing the fluorescent dye,
propidium iodide, and the measurement chamber.