ChemoMetec YC-100 - Manual (Page 25)

2 Introducing the NucleoCounter
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by multiplying the imaged area with the depth of the cassette. The imaged area is only
dependent on the optics of the instrument. Therefore, the area is constant and specific
for each instrument.
2.2.4 Cassette handling
The built-in image analysis method of the NucleoCounter offers considerable stability,
when eliminating non-cellular objects from the image, including scratches and smears
on the cassette windows, and thus producing valid results even under extreme
conditions. On the other hand, the quality of the cell count is best assured by keeping
the windows of the cassette as clean as possible.
Caution! In order to avoid contaminating the measurement window it is important not
to touch the window when handling the cassettes. Be careful when attempting to wipe
off the surface of the cassette, in an attempt to remove any foreign object, since the
plastic material of the cassette can be scratched.
2.3 Lysis buffer
The lysis buffer for the lysing of yeast cells, Reagent Y100, is suitable for the sample
preparation of majority of yeast cell suspensions. It is highly effective and generally
achieves instantaneous lysing of yeast cells in suspension. Nevertheless certain species
of yeast cells or samples might require different preparation. Thus if you encounter
problems relating to insufficient lysing of yeast cells, please contact ChemoMetec A/S
for assistance.