ChemoMetec YC-100 - Manual (Page 35)

5 Operation of the NucleoCounter
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Contrast? 6 Contrast? 6 Contrast? 6 Contrast? 6
1: Up 1: Up 1: Up 1: Up
2: Down 2: Down 2: Down 2: Down
↵ ↵ ↵ ↵ store store store store
Figure Figure Figure Figure 16 16 16 16.... LCD-contrast value 6 is going to be chosen
Press “↵ ↵ ↵ ↵ ” to store the chosen value (or “Esc” Esc” Esc” Esc” to return to the previous value).
If the value has been changed the new contrast will be remembered during the session.
When shutting down the NucleoCounter asks if the new value shall be saved as the new
default value. Press “↵
↵ ↵ ↵ ” to save or “Esc Esc Esc Esc” if the present default value should not be
overwritten.
5.4.6 Instrument Info (F100)
When typing “F + 1 + 0 + 0 + F + 1 + 0 + 0 + F + 1 + 0 + 0 + F + 1 + 0 + 0 + ↵ ↵ ↵ ↵ ” the Instrument Info will appear in the display as in the
Display Text Type shown below.
ChemoMetec A/S ChemoMetec A/S ChemoMetec A/S ChemoMetec A/S
NC NC NC NC YC YC YC YC- - - -100 100 100 100
V V V V4 4 4 4. . . .xx xx xx xx 200 200 200 2005 5 5 5- - - -MM MM MM MM- - - -DD DD DD DD
S/N: 00 S/N: 00 S/N: 00 S/N: 002 2 2 2- - - -01 01 01 01
Figure Figure Figure Figure 17 17 17 17.... Instrument Info
The display shows the name of the instrument in line 1 of the display and the
instrument type in line 2 of the display. The version number and date (year-month-
date) of the integral software of the instrument is shown in line 3. The serial number of
the instrument is shown in line 4 of the display. The display text is almost identical to
the start-up display text (Figure 8).
5.5 Instrument Sensitivity (F11)
The NucleoCounter YC-100 detects signals from propidium iodide which intercalates with
double stranded DNA within the cell. Due to a variety of factors, such as strain,
environment, growth phase and haploid/diploid forms the detected signal from yeast
cells can vary. To allow the user to compensate for this it is possible to change the
select different integration time for the CCD camera used to collect the fluorescent
image. This is done by the user function F11, which is activated by typing “
F11 + ↵ ↵ ↵ ↵ ”.
Then the following dialogue will appear: