Luminex xPONENT for MAGPIX 4.2 - Manual (Page 54)

• Analysis Settings - Displays the analysis type to be used for the batch. Use this section to
set the analysis type, set the number of standards and controls, select an external analysis
program, and choose whether or not to analyze results while acquiring samples.
Analysis Type - Use this list to choose from the following analysis types:
• None - No analysis. Select if you have your own data post-processing program and
want to obtain only fluorescent intensity results. You cannot apply standards or
controls when you select None. You cannot analyze acquisitions with this setting.
• Qualitative - Qualitative analysis determines results as either positive or negative,
reactive or non-reactive. The software is flexible in defining custom result ranges,
such as negative, low positive, or high positive. Determinations are based on a single
standard. For qualitative analysis the Luminex software uses a specific algorithm,
shown below.
(FIsample)/(FIstandard) = Ki
Where FI = Fluorescent Intensity and Ki = a “Quali” value entered in lot information to
determine the value or the qualitative assay standard.
• The “Quali” value determines a cutoff or threshold. This, in conjunction with ranges
using the Lum Qual formula or an edited range specific for your assay, helps to
determine qualitative results for unknown samples.
• Two predefined formulas using the algorithm are included in the system. You can use
them as is or edit their range values to meet your requirements.
• Quantitative - Determines the sample concentrations from standard curves using
regression methods Cubic Spline, Linear, Logistic 4P, and Logistic 5P, Luminex
Logistic 4P, and Luminex Logistic 5P. Type the desired values for standards and
controls in the Number of Standards and Number of Controls boxes. Select either
Fit of All Standards or Mean of Replicates for the calculation of the curve fit.
NOTE: Luminex recommends Fit of All Standards as the most accurate
calculation of the curve fit .
Based on a range of quantitative, numerical results, a threshold range can be applied
to a quantitative analysis, for example, high, low, saturated, and expected.
• Allele Call - Sets analysis for an allele call. Analytes must be put in groups of 2, 3, or
4 analytes.
• Min MFI Enabled - Select this box to enable a minimum MFI for the Allele Call
analysis. Type a value in this box to set the minimum MFI for analysis.
• Number of Standards - Click to type the number of standards for the protocol.
• Number of Controls - Click to type the number of controls for the protocol.
• Fit of all Standards - The standard curve is determined by using each individual
standard replicate when calculating the standard curve. For example, if you run
duplicates of a 7 point standard curve, the software will calculate the standard curve
by using 14 points.
• Mean of Replicates - The standard curve will be determined by averaging the
individual standard replicates when calculating the standard curve. For example, if
you run duplicates of a 7-point standard curve, the software will calculate the standard
curve by using 7 averaged points.
• Analyze Results While Acquiring Samples - The software allows for real-time
viewing of the results as the instrument analyzes samples.
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