Luminex xPONENT for MAGPIX 4.2 - Manual (Page 58)

• Selected Analytes list - Selected analytes are displayed in a list on the right side of the
analyte grid. This list includes the following information:
• Name - The name of the analyte. Click and type to rename the analyte.
• Analysis - To change the type of analysis for an analyte, click this field to open the
Analysis Settings dialog box and select another analysis from the list.
In the Analysis Settings dialog box:
FIGURE 4. Analysis Settings Dialog Box
1. Select a method from the Method list.
2. If necessary, select a weight type from the Weight Type list.
3. Apply the analysis to all analytes in the list by clicking Apply to All Analytes.
4. Select Mark as Intra-Well Normalization Bead to make the analyte an intra-well
normalization bead. The normalization bead is a microsphere set that is included in
the assay as an internal control. It controls for sample variation and can be used to
normalize data between samples in a run.
5. Add a range to the analysis by clicking Add Range.
6. Select Use Threshold Ranges to enable ranges for the analysis.
7. Click Add Range to add a range.
8. Type a Range Name, a Low Value, a High Value, and select Inclusive if you want
to include the low and high values in the range. Click OK to exit the dialog box.
• Units - The unit of measurement you specified in the Unit box. Click this box to type a
value for the analyte.
• Count - Type the desired bead count for the analytes by clicking in the Count box. If
each selected bead set does not acquire this number of events, a warning is added to
the log that not enough bead events were acquired.
• Region - Refers to the specific analyte selected. This is a number between 12 and 78.
• Group - If you have selected Allele Call from the Analysis Type in the Settings tab, this
button is displayed. Click Group to group 2, 3, or 4 analytes for the group. Multiple groups
can be defined.
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