Luminex xPONENT for MAGPIX 4.2 - Manual (Page 93)

13. To set a bead count and the units for a single analyte, click in the Units and Count
columns directly to the right of the analyte grid, and type a bead count and units value.
14. To change the default analysis for all analytes, click Change. The Analysis Settings
dialog box opens.
15. In the Analysis Settings dialog box, select the analysis method from the Method list,
and the weighting in the Weight Type list. Click Apply to All Analytes to apply the
selection to all analytes.
16. To change the analysis for a single analyte, click the Analysis field for the analyte you
want to modify. The Analysis Settings dialog box opens.
17. Select an analysis method in the Method list.
18. Select a weight type in the Weight Type list (Weight Type may not display, depending
on the analysis method selected in the Method list).
19. Check Mark as Intra-Well Normalization Bead if you want to use the analyte as a
normalization bead. The normalization bead is a microsphere set that is included in the
assay as an internal control. It controls for sample variation and can be used to normalize
data between samples in a run.
20. Check Use Threshold Ranges if you want to use a range for the analysis.
21. Click Add Range to setup the threshold range.
22. Type a name for the range in the Range Name field.
23. Type low and high range values in the Low Value and High Value fields.
24. Select the check box in the Inclusive column to include the value in the range, or leave it
clear to make the range value one unit higher than the low value, and one unit lower than
the high value.
25. Highlight a range and click Delete Range to delete the range.
26. Click OK to apply the new settings to the analyte first clicked, or Apply to All Analytes to
apply them to all the analytes in the protocol.
xPONENT for MAGPIX 4.2 Software User Manual
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