Leica Biosystems CM3050 S User Manual
Leica cm3050 s, Cryostat
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Table of contents
Document Outline
- 1. Important information
- 1.1 Symbols used in this manual and their meaning
- 1.2 Designated use
- 1.3 Qualification of personnel
- 2. Safety
- 2.1 General information on instrument design and safe handling
- 2.2 Integrated safety devices
- 2.2.1 Locking the handwheel
- 2.2.2 Centering the handwheel grip
- 2.2.3 Emergency stop function (instruments with sectioning motor only)
- 2.2.4 Knife guard
- 2.3 Safety instructions: handling and operation of the instrument
- 2.3.1 Transport
- 2.3.2 Site requirements
- 2.3.3 Electrical connections
- 2.3.4 Handling microtome knives/blades
- 2.3.5 Knife guard/handwheel lock
- 2.3.6 Motorized sectioning
- 2.3.7 Defrosting/Handling frozen tissue
- 2.3.8 Frozen parts of the instrument and frozen accessories
- 2.3.9 Infectious/radioactive material
- 2.3.10 Disinfection and cleaning
- 2.3.11 Removing/reinstalling the microtome
- 2.3.12 Display message ‘Dry microtome’
- 2.3.13 Maintenance
- 3. Installation
- 3.1 Site requirements
- 3.1.1 General site requirements
- 3.1.2 Electrical connections
- 3.2 Unpacking and installation
- 3.2.1 Repacking
- 3.3 Standard delivery
- 3.4 Installing/inserting accessories
- 3.4.1 Installing the handwheel
- 3.4.2 Inserting the accessories
- 3.4.3 The footswitch
- 3.5 Prior to switching on the instrument
- 4. Instrument Properties
- 4.1 Overview
- 4.2 Technical Data
- 5. Operation
- 5.1 Operating the instrument for the first time
- 5.2 Switching on/fuses
- 5.3 Control panel 1
- 5.3.1 Key functions in control panel 1
- 5.3.2 Display functions in control panel 1
- 5.3.3 Display indications when switching on
- 5.3.4 Menu functions: setting parameters of refrigeration, time and preset counter
- 5.3.5 Status display
- 5.3.6 Indication of refrigeration state, section thickness setting
- and counters
- 5.4 Control panel 2
- 5.4.1 Selecting a sectioning mode /
- start and stop sectioning
- 5.4.2 Setting the sectioning window
- 5.4.3 Selecting sectioning speed
- 5.4.4 Emergency stop
- 5.4.5 Coarse feed keys
- 5.4.6 Trimming and sectioning functionsswitching between trimming and sectioning
- 5.4.7 Setting the section thickness
- 5.5 Daily operation of the instrument
- 5.5.1 Freezing specimens onto specimen discs with quick-freeze shelf and stationary heat extractor
- 5.5.2 Freezing specimens onto specimen discs with 90 ° prism (Instruments w/specimen cooling only)
- 5.5.3 Optional accessories for freezing specimens onto specimen discs and for removing frozen specimens
- 5.5.4 Inserting specimen discs into the specimen head
- 5.5.5 Specimen orientation
- 5.5.6 Trimming / sectioning
- 5.6 Finishing work
- 5.6.1 Daily routine steps
- 5.6.2 Switching off the instrument for a longer period of time
- 5.7 Defrosting
- 5.7.1 Automatic chamber defrost cycle
- 5.7.2 Manual chamber defrost cycle
- 5.7.3 Defrosting the specimen head (instruments with specimen cooling only)
- 6. Troubleshooting, applications tips
- 6.1 Displayed error messages
- 6.1.1 Other error indications
- 6.2 Potential problems - causes and remedies
- 6.3 Reference chart for temperature settings (in minus °C)
- 7. Disinfection, cleaning and maintenance
- 7.1 Safety instructions on disinfection and cleaning
- 7.2 Spray disinfection with Leica Cryofect
- 7.3 Disinfection with conventional disinfectants
- 7.4 Cleaning
- 7.5 Removing / reinstalling the microtome
- 7.5.1 Removing the microtome
- 7.5.2 Disassembling the specimen head (instruments w/specimen cooling only)
- 7.5.3 Reinstalling the microtome
- 7.6 Exchanging the fluorescent light lamp
- 8. Warranty and service
- 9. Appendix
- 9.1 Electrohydraulic height adjustment
- 9.1.1 Designated use
- 9.1.2 Standard delivery
- 9.1.3 Safety instructions
- 9.1.4 Installation
- 9.1.5 Control keys
- 9.1.6 Maintenance and care
- 9.1.7 Troubleshooting
- 9.1.8 Technical Data
- 9.2 Ordering information - accessories
- 10. Decontamination Certificate (master)
- 11. Information for the People´s Republik of China