SciGene 700 Microarray Oven User Manual

Page 8

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Model 700 Microarray Oven — USER MANUAL

Version 2.1, February 2011 (408) 733-7337, [email protected]

7

F. Calibrating the Oven Temperature

To ensure accurate (±0.5°C) oven temperatures, the performance of the
Watlow EZ-Zone controller should be periodically checked by comparing
the temperature displayed on the controller to an accurate, NIST-
certified (National Institute of Standards and Technology) thermal
measurement device. Calibration need only be performed at a single
temperature (e.g. 65°C) to achieve accurate oven operating temperatures
from ambient +5 to 99°C.

SciGene recommends the calibration method below to achieve
accurate oven temperatures. No other methods are recommended.

1. Calibration Check

a.

Turn on the oven, set to 65°C and allow temperature to

stabilize (30 min).

b.

Connect a NIST-certified digital thermometer (SciGene Cat.

#1051-52-0) to the blue jack (secondary RTD) at the top back
of the oven.

c.

Verify that the temperature shown on the digital thermometer

matches that shown on the controller within ±0.5°C. If it does
not, follow the steps below to adjust the controller.

2. Calibration Procedure

a.

Turn on the oven, set to 65°C and allow temperature to

stabilize (30 min).

b.

Connect a NIST-certified digital thermometer (SciGene Cat.

#1051-52-0) to the blue jack at the top back of the oven.

c.

Calculate the difference in temperature shown on the

controller to the digital thermometer. For example, if the
controller displays 65.0°C and the thermometer displays
66.5°C, the difference (offset) is 1.5°C.

d.

On the controller, press the up and down arrows

simultaneously for 3 seconds. The upper display will now read
“A1” and the lower display “open”.

e.

Press the green circle button 3 times until the lower display

shows “i.CA”. The upper display shows the offset value
between the controller and thermometer when the unit was
last calibrated.

f.

Use the up/down arrows to adjust the offset value to the

temperature difference calculated in Step c. For example, if the
controller displays a temperature 1.5°C lower than the
thermometer, adjust the offset by adding 1.5 to the value shown.

g.

Press the

key twice to return to the operation display. Your

oven is now calibrated to provide accurate temperatures
between ambient + 5 to 99°C.

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