Operation – UVP EC3 Imaging System User Manual

Page 6

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Operation

1.

Turn on the master power switch on the front of the darkroom cabinet.
Once the power is switched on, power is applied to all internal
components and to the power port for the jumper power cable that
supplies power to the transilluminator.

2.

The Transilluminator Control knob has five options:

White Light On - Operates the white light plate located on the platform

UV Safety - Transilluminator safety control switch automatically turns
the transilluminator off when door opens and prevents the user
from exposure to UV

UV Always Off - This button setting puts the transilluminator in Off
mode. Use this switch when the transilluminator is not

required

such as when capturing chemi blots.

UV Always On - This setting overrides the safety switch with the
transilluminator always in the on mode. Users should wear
protective clothing and eyewear when the darkroom is in the
Always On mode to prevent exposure to the UV
.

Timer Switch - The timer operates the transilluminator. It is
automatically set to shut off at 10 minutes to reduce overexposure to
samples. To override this function, push the timer button to off or
press reset to start the timer again.

3.

The Overhead Light control knob has four options for providing
overhead diffused 254 and 365nm UV and fluorescent white light for
uniform epi-illumination.

Off - Place the overhead light switch in the Off mode when not using
the darkroom for applications requiring epi-illumination.

White - Overhead white light illuminates when “white” is selected.

365nm and 254nm - When either ultraviolet wavelength is selected,
the red light will illuminate to alert the user that the light is in operation.
The overhead lights are designed to automatically turn off when the
darkroom door is open.

4.

The Emission Filter Wheel is designed with five-positions for
viewing samples with the 400 - 655nm emission filters. Use the
interface center to select from:

Blue (400-600nm)

Green (515-570nm)

Gold (485-655nm)

Red (570-640nm)

Clear (empty)

4

EC3 Imaging System

Emission Filter Wheel Control

Overhead Light Control

Transillumination Controls

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