UVP FirstLight UV Transilluminators - Manual (Page 4)

FirstLight UV Transilluminators Page 4
When the UV Blocking Cover is not being
used, UV light may escape through the
holes dedicated to accepting the bracket
pins of the UV Blocking Cover.

Remove the black safety plugs from
their package

Insert the safety plugs through the
holes as shown.
Set-Up

Place the transilluminator on a level work surface. Be sure that an air space exists around the
bottom of the work surface. This space allows for the proper air circulation through the unit.

Plug the female end of the power cord into the transilluminator. For 230 volt models, or those
requiring special power cord connectors, ensure that the proper configuration of male
connector or plug has been properly connected to the power cord.

Plug the male end of the power cord into a properly grounded electrical outlet. The proper
voltage of the transilluminator is found on the product information label.
NOTE: If using the transilluminator with an imaging system, a jumper cable is required for
connecting to the darkroom. Refer to the imaging system documentation for additional
instructions.

The transilluminator may be equipped with a UV Blocking Cover. Remove the brown protective
paper from the cover. Insert the bracket pins on the cover into the holes on the front of the
transilluminator. The cover is adjustable to varying angles for access to the filter surface.
If not using the transilluminator with an imaging system darkroom, do not operate the unit without
securing the cover. If the cover is missing, a UV Blocking Faceshield must be worn to avoid UV
exposure to the skin. UV Blocking Eyewear should be worn even with the cover in place to avoid
accidental UV exposure.
FirstLight Applications
Using the FirstLight along with digital fluorescent CCD imaging has a number of advantages,
including:

Low capital cost compared to laser based scanning

High detection sensitivity

Wide dynamic range

Rapid signal acquisition by low noise CCD cameras (typically msec to seconds)

Availability of a wide range of highly sensitive stains for protein and nucleic acid analysis. With
few exceptions, fluorescent stains used in post electrophoresis analysis of proteins and nucleic
acids have significant excitation peaks with ultraviolet (300-365 nm) light, making midrange UV
the excitation source of choice for high sensitivity analysis for most fluorophores [2].

Rapid multiplex analysis of proteins (multiple fluorescent signatures from a single gel), greatly
simplifying the analysis of protein expression, turnover, and posttranslational modifications
after one and two dimensional SDS PAGE separations.
Safety Plug