UVP FirstLight UV Transilluminators - Manual (Page 5)

FirstLight UV Transilluminators Page 5
In the past, quantitative CCD imaging with UV has been difficult due to the lack of uniformity found
in typical UV transilluminators. Accurate and repeatable UV imaging requires a uniform light
source. Uniform Illumination of the FirstLight is critical for quantitative analysis and ensures:

Sensitivity and dynamic range are consistent across the illumination surface

Little or no reliance on uniformity correction by software that can lead to low signal data loss

Straightforward gel to gel comparison
Through design of the FirstLight UV Illuminator, quantitative UV imaging is now possible.
Using the Transilluminator

Place gel/sample on the filter area. It is recommended to place the gels on a Gel-Tray to
protect the filter surface from cuts and scratches. It is recommended that gloves be worn to
avoid contact with gel and staining agents.

Press the ON/OFF switch to ON. Prior to use each day, it is recommended that approximately
a ten-minute warm-up period be observed for the initial use. When frequently turning the unit
on and off, the warm-up period is not required.

After viewing/photographing the sample, turn the transilluminator off.
Uniformity
Through the use of a high density lighting system with a tuned phosphor coating, uniformity of <5%
CV across the imaging surface is achieved and applied for quantiative fluorescent imaging.
Each unit comes with a Certificate of Uniformity, measuring the coefficent of Variance.
The chart below demonstrates the uniformity across the filter area.