21 conditioning – Metrohm 940 Professional IC Vario ONE/ChS User Manual

Page 58

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3.21 Conditioning

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940 Professional IC Vario ONE/ChS (2.940.1200)

5 Installing the output of the separation column

Fasten the column output capillary to the upper end of the sepa-
ration column using a PEEK pressure screw (6.2744.070).

6 Inserting the separation column

Insert the separation column with chip into the column holder
until you hear it snap in place.

The separation column is now detected by MagIC Net.

3.21

Conditioning

After installation, after each time the instrument is switched on and after
each time the eluent is changed, the system has to be conditioned with
eluent long enough that a stable baseline is attained.

NOTE

The conditioning time can lengthen considerably after changing the
eluent.

Conditioning the system

1 Preparing the software

CAUTION

Ensure that the configured flow is not higher than the flow permit-
ted for the corresponding column (refer to the column leaflet and
the chip data record).

Start the MagIC Net computer program.

Open the Equilibration tab in MagIC Net: Work-
place

Run Equilibration.

Select (or create) a suitable method.
Also see: MagIC Net tutorial and online help.

2 Preparing the instrument

Ensure that the column is inserted correctly in relation to the flow
direction marked on the sticker (arrow has to point in the direc-
tion of flow).

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