3 installation diagram, Installation diagram – Metrohm 850 Professional IC Cation - Gradient User Manual

Page 22

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3.3 Installation diagram

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850 Professional IC Cation – HP-Gradient

Set contact pressure of the peristaltic pump .

Rinse instrument without column.

7 Connecting the columns

Remove the 6.2744.040 coupling between column inlet capillaries
and detector inlet capillaries.

Connect guard column (optional) (see Chapter 3.17, page 47)

– Fasten the guard column to the end of the column inlet

capillary according to the specifications contained in the
leaflet accompanying the guard column.

– Rinse guard column.

Connect the separation column (see Chapter 3.18, page 48)

– Fasten the inlet of the separation column either to the end

of the column inlet capillary or to the guard column (if
used) according to the specifications contained in the leaf-
let accompanying the column.

– Rinse separation column.
– Connect the detector inlet capillary and the output of the

separation column using a 6.2744.070 short PEEK pressure
screw.

8 Conditioning the instrument

See Chapter , Page 51.

The instrument is now ready for measuring samples.

3.3

Installation diagram

Figure 3 Installation diagram shows the capillary connections with the
application of a a binary high pressure gradient.

The arrangement of the modules in the diagram corresponds to the front
view of the instrument. Liquid containers (eluent bottle, sample vessel,
waste container, auxiliary solutions container) and guard column are not
shown in the diagram.

Most of the capillaries are already pre-installed at the time the instrument
is delivered. Capillaries on which nothing needs to be done at the time of
initial installation are not numbered in the diagram.

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